Introduction
This protocol utilizes a multi-modal pharmacological approach to address age-related degradation of the integumentary system. By combining regenerative signaling peptides and growth hormone secretagogues, this regimen aims to restore the structural integrity of the extracellular matrix (ECM) from a systemic level. The mechanism of action centers on fibroblast activation, angiogenesis, the upregulation of collagen and elastin synthesis and addressing systemic inflammation common with those seeking a more youthful appearance. Unlike topical treatments which are limited by the stratum corneum, this protocol delivers bioactive agents primarily via subcutaneous injections to the vascular and cellular architecture supporting hair, skin, and nails. This protocol relies primarily on a subcutaneous delivery approach, making self-administration easier and more practical. Those implementing this protocol should view it as a restorative intervention for connective tissue health, rather than a purely cosmetic procedure.
Peptide Agents & Compounds
- GHK-Cu (Tripeptide-1): A naturally occurring copper-binding peptide that modulates collagen synthesis and breakdown. It functions as a feedback signal for tissue remodeling and possesses potent anti-inflammatory and antioxidant properties necessary for skin regeneration.
- BPC-157 (Body Protection Compound): A pentadecapeptide derived from gastric juice that accelerates soft tissue healing. Its primary utility in this protocol is the stimulation of angiogenesis (VEGF expression) to improve blood flow to the dermal matrix and hair follicles.
- TB-500 (Thymosin Beta-4 Fragment): A synthetic fraction of the protein thymosin beta-4 which promotes endothelial cell migration and differentiation. It works synergistically with BPC-157 to repair damaged tissue and reduce inflammation at the cellular level.
- KPV (Lysine-Proline-Valine): KPV exerts significant anti-inflammatory effects, making it particularly indicated for patients with inflammatory dermatoses or compromised skin barriers, which works synergistically with GHK-Cu in strengthening the skin barrier, calming skin irritation, and diminishing signs of aging.
- NAD+ (Nicotinamide Adenine Dinucleotide): A critical coenzyme in poly-ADP-ribose polymerases (PARP) and sirtuin (SIRT1) activation. PARP1 consumes NAD+ to maintain genomic integrity by influencing DNA repair, gene transcription, and chromatin structure. By supplementing with NAD+, improvements in the age-related decline of energy levels related to low intracellular NAD+ should be seen quickly by patients.
- CJC-1295 with DAC: A long-acting Growth Hormone Releasing Hormone (GHRH) analog with Drug Affinity Complex (DAC) to extend half-life, making it a more convenient solution for increasing endogenous Growth Hormone release. It provides sustained elevation of plasma Growth Hormone and IGF-1 levels, driving protein synthesis and systemic tissue repair.
- Epitalon (Epithalon): A synthetic tetrapeptide that mimics pineal gland secretion. Its inclusion addresses cellular senescence by stimulating telomerase activity in fibroblasts, thereby extending the replicative capacity of skin cells. Epitalon also has a profound genomic effect on the circadian rhythm, improving both the quality of sleep and sleep patterns.
- Topical Bioactive Serum/Cream and Microneedling: Monthly microneedling coupled with daily application of a Serum and Cream containing GHK-Cu, Argireline, Matrixyl, SNAP-8, and/or Tranexamic Acid serves as a direct adjunct to the systemic therapy of GHK-Cu, often targeting surface-level hyperpigmentation, fine lines, and wrinkles.
Oral Supplementation
- Zinc Picolinate or Glycinate: Essential for maintaining metallothionein balance while increasing systemic Copper levels via GHK-Cu administration. Zinc acts as a competitive antagonist to copper absorption and is a critical cofactor for keratin production.
- Hydrolyzed Collagen Peptides: Provides the requisite amino acid substrate (specifically glycine, proline, and hydroxyproline). High-dose exogenous collagen ensures that the upregulation of synthesis pathways triggered by peptides is met with sufficient raw material.
Potential Benefits
Outcomes one can expect by implementing this protocol is a measurable increase in dermal thickness and elasticity due to enhanced collagen I and III production. The angiogenic properties of the regenerative peptides result in improved microcirculation, presenting as enhanced hair follicle density, improved skin dermal resilience/repair, and accelerated nail growth rates. Reducing oxidative stress markers and improve epithelial stem cell efficiency slows the effects of aging seen in skin tissue, particularly on areas exposed to more environmental damage. Secondary benefits often include improved joint lubrication and soft tissue recovery due to the systemic nature of VEGF and Growth Hormone modulation.
Protocol Dosing Schedule
1. GHK-Cu: Initiate dosing at 0.5mg daily via subcutaneous administration. Titrate up every 4-7 days by 0.5mg as tolerated, to a maximum of dose of 2.0mg daily. Due to its acidity and composition, storing reconstituted GHK-Cu with other peptides or compounds long-term could cause degradation, rendering all compounds inert; only combine reconstituted GHK-Cu with other compounds in the syringe immediately before administration. If a significant allergic reaction occurs at the injection site, recommend the use of an antihistamine, such as loratadine or cetirizine, to counteract the histamine reaction.
2. Regenerative Blend (BPC-157 / TB-500 / KPV): Dosage for each peptide is the same and weight-dependent, ranging from 100mcg to 600mcg per 100lbs daily via a subcutaneous injection; for anti-aging, 200mcg/100lbs is common. There is limited data to suggest if any of these compounds should be cycled; however, a cautious approach would be to administer the peptides (individually or combined) for a maximum duration of 12 weeks with at least 4 weeks. Given the complimentary pathways modulated by each compound on inflammation and angiogenesis, it’s best to assess the needs of the individual when determining how to best incorporate each into an anti-longevity protocol. These compounds can be administered at the same time and in the same syringe as GHK-Cu to neutralize the pH level of both solutions and improve injection comfort.
3. CJC-1295 with DAC: Dosing is 1mg subcutaneously every 3-4 days. Given the effects DAC has on the enzymatic degradation of the GHRH analog, allergic reactions have been noted. Also, monitor for transient water retention, which could persist up to 3 weeks for men and 6 weeks for women. CJC-1295 with DAC may be co-administered with a daily administration of Ipamorelin (up to 900mcg per day) if additional pulsatile release is indicated.
4. NAD+ (Subcutaneous): Dosing starts with a 25mg subcutaneous injection administered every 3-4 days. Then, the dosing titrates up by 25mg every two weeks, up to a maximum tolerated dose of 100mg per dose every 3-4 days. A loading dose of 100mg-250mg administered via an IV injection is strongly recommended to initiate the protocol to minimize side effects for those with low intracellular NAD+ levels. Monitor for anxiolytic side effects as well as elevated liver enzymes. Given NAD+ composition, it is not recommended to combine with any other compounds during administration.
5. Epitalon: Dosing is up to 10mg daily via subcutaneous injection for 5 to 10 days until a total of 50mg per cycle is completed. This administration protocol should be repeated every 4 months.
6. Topical Application & Microneedling: Apply a bioactive serum and cream daily to the area of the body where a reduction in fine lines and wrinkles are desired or there’s a desire to stimulate the growth of more hair. Every 4-6 weeks, the patient should complete a microneedling session with hyaluronic acid, peptides, and human growth factors, exosomes, and/or stem cells to bypass the stratum corneum and enhance dermal penetration.
Potential Side Effects & Contraindications
Fluid Retention (Edema): The use of CJC-1295 with DAC is associated with transient water retention, manifesting as extremity edema or carpal tunnel-like symptoms. This is a physiological adaptation phase typically exacerbated by chronic dehydration and/or excessive salt/electrolyte consume. This typically resolves within 3 weeks for males and 6 weeks for females.
Injection Site Reactions: GHK-Cu may cause localized pain, redness, or induration post-injection due to its pH level and/or allergic reaction caused by histidine. Mixing GHK-Cu with PBS (phosphate buffered saline, 7.2-7.4), 2 parts PBS to 1 part GHK-Cu solution, immediately prior to injection mitigates the initial ISR caused by contrasting pH levels. To mitigate the immune response, combine the GHK-Cu solution with the some/all of the peptides in the regenerative blend and ensure a slow titration schedule.
Zinc/Copper Imbalance: Failure to adhere to zinc supplementation may lead to copper dominance, presenting as fatigue or mood lability. Regular monitoring of serum copper and zinc levels is advised.
Contraindications: This protocol is contraindicated in patients with active malignancies, as angiogenesis and growth factor upregulation have been shown to accelerate tumor and cancer cell growth. It is also contraindicated during pregnancy and breastfeeding to start this protocol, as the in utero effects of these compounds are unknown.
Expected Outcomes
Clinical observation suggests initial improvements in skin texture and hydration within 4-6 weeks of adherence. Significant structural changes, such as hair density improvement and reduction in deep rhytids, typically require 3-6 months of continuous therapy. Patients should be counseled that efficacy is dependent on substrate availability; therefore, adherence to the oral supplementation and hydration protocols are mandatory for optimal results.
Conclusion
This Anti-Aging Protocol represents a sophisticated application of regenerative medicine, targeting the cellular mechanisms of aging rather than merely masking symptoms. By integrating systemic peptide therapy with targeted dermal remodeling, physicians can offer a comprehensive solution for aesthetic rejuvenation. Success relies on careful patient monitoring, particularly during the titration and adaptation phases, ensuring that hormonal and mineral balances are maintained throughout the treatment course.